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Next morning, the stained gel was de-stained by replacing coommassie blue staining solution with the de-stained solution I (40% (v/v) methanol, 7% '(v/v) acetic acid), and shaked slowly for 30 min. This removed the buck of the excess stain.
JC1 staining solution was prepared by diluting the stock in culture medium at 1 10 dilution ratio.
SYBRGold gel staining solution was purchased from Molecular Probes Inc. (Oregon, USA).
Following treatment, cells were stained with a crystal violet staining solution [46].
An additional 10 µl of staining solution was added and mounted with a coverslip.
The samples were incubated in GUS staining solution at 37°C overnight.
The seedlings were then immersed in GUS staining solution and treated as described above.
After an incubation time of 55 seconds, further 300 µl of staining solution were added.
Explants were then assayed for β-gal activity by staining with XGal staining solution overnight at 37°C.
Cell viability was tested using 7AAD viability staining solution (eBioscience).
DAPI staining solution was purchased from Beyotime Institute of Biotechnology (China).
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