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The tavern is literally covered with them -- shamrocks in different shades of green, many fading into a dark, mustard brown, stained over the years by clouds of cigarette smoke.
Cells were stained over night at 4°C for CD133 (1/100) (polyclonal antibody, Abcam), GFAP (1/300) (monoclonal antibody, R&D Systems), myogenin (1/2) (F5D; monoclonal antibody, Developmental Studies Hybridoma bank) and N-CAM (1/2) (5.1H11; Developmental Studies Hybridoma bank)).
Germinated pollen was stained over night at 37°C with 1 mg/ml X-Gluc solution containing 50 mM Na3PO4, 0.5 mM K3Fe CN 6, 0.5 mM K4Fe CN 6, 10 mM EDTA, 0.01% Triton X-100 and 10% (w/v) sucrose.
In prostate adenocarcinomas, LS-A2021 stained over 50% (17/29) of tissue cores while LS-A2019 stained over ~30% (10/29).
Cells were stained over night at 4 °C for NES (196908) (MAB1259, R&D Systems, Minneapolis, MN, USA) and TUBB3 (Abcam78, Abcam).
For each study group, the positivity index for each staining was calculated as the ratio of specimens being strongly or intermediately stained over the sample population.
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Evidence of positive staining was recorded as presence of staining (yes/no) or percent of epithelial or basal cells staining (number of cells staining over total number of cells).
However, reprogrammed cells lose striated sarcomere staining over time.
However, we detected no evidence of degradation of TH staining over time, even in experiments exceeding 100 minutes (Figure 1C,D).
The result showed that the relative pS935 levels, as measured with anti-pS935 staining over anti-FLAG staining, were significantly higher with co-transfection of Myc-14-3-3γ Myc-14-3-3γ Myc-14-3-3γ Myc-14-3-3γsfection andne.
In <10% of cells we also saw staining over the acrosome.
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