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Membranes were stained reversibly stained (kit) to confirm homogeneity of protein loading, and cut in separate strips.
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b g Fluorescent images of 4T1 cells stained with a Hoechst/PI double staining kit.
Tregs were analyzed by using mouse regulatory T cell Staining Kit (eBioscience).
An apoptosis Hoechst 33258 fluorescence staining kit was provided by Beyotime Institute Biotechnology (Shanghai, China).
Flow cytometric analysis of apoptotic cells was performed using an Annexin V-FITC/PI staining kit (BD Biosciences, CA, USA).
Fluorescein Active caspase-9 staining kit was purchased from Biovision.
The live-dead fixable dead cells stain kit was obtained from Invitrogen (Carlsbad, CA).
The reaction product was developed with DAKO DAB staining kit, dehydrated and mounted with DPX.
Foxp3 expression was measured by Flow Cytometry using intracellular Foxp3 staining kit from eBioscience, USA.
Avidin-biotic complex (ABC) staining kit for immunohistochemistry was from Vector Laboratories (Burlinghame, CA).
Secondary antibody staining kit was purchased from Dako.
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