To address this question, we coexpressed a Flag-Sac1 transgene in ey-Gal4,DVAP-P58S recombinant flies and stained eye imaginal discs with antibodies specific for Flag and DVAP.
To determine the localization of the three USH1 orthologs Sans, Cad99C, and Crinkled/Myosin VIIa in the Drosophila eye, we stained adult eyes with antisera specific to these proteins.
For whole mount retinal immunofluorescence staining, eyes were fixed in 4% paraformaldehyde overnight.
For Hematoxylin and Eosin (H&E) staining, eyes were removed and eyecups were fixed in 4% paraformaldehyde for 2 h, washed three times with PBS, and embedded in paraffin.
For H&E staining, eyes were fixed in 4% PFA overnight, dehydrated, and embedded in paraffin.
For immunohistochemical (IHC) staining, eyes were frozen immediately in liquid nitrogen; 4-μm cryosections were placed on glass slides and maintained at –80°C until analysis.
Those bloody ink blots staining her eyes were the mark of the train that had knocked her flat on her face between the tracks, swiftly and precisely enough to keep her alive.
For morphometric analysis, the middle sections of the eyes stained by methylene blue and Basic fuchsin were used to measure eye size, lens size and the depth of anterior chamber at E15.5, E17.5 and P21.
If you're leaving make-up stains (eye or otherwise) on your pillows, use a soft makeup remover, too.
Their faces were stained and their eyes unfocused.
