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Invaded cells were stained, collected and lysed according to the manufacturer's instructions.
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Flow cytometric analysis of apoptosis was performed after staining collected cells with annexin-V FITC)/annexin-V FITCI(PC5) and annexin-V(FITC)/anti-CD4 PE /PI PC55).
Marker biotinylated Peanut Agglutinin (PNA, Vector Laboratories) was used to stain collecting ducts.
For example, an old stain collected at the scene of an unsolved case of a robbery can be matched to the profile of an individual who is presently being charged or convicted for other crimes.
The samples available included a biological stain collected on the body of the victim and the DNA of three suspects (Fig. 3).
Forensic mixed stain collected on the body of the victim and reference samples from the victim (blood) and the three suspects (saliva) were extracted with the QIAamp DNA mini kit following manufacture's instruction.
Inflammation scores of H&E stained tissue collected 7 days post operation.
d Inflammation scores in H&E stained tissue collected 7 days post operation.
In this case, all viable cells (i.e. non-PI stained) were collected by FACS for RNA isolation.
We stained PBMCs collected before, and at multiple time points up to 1 year after MVA85A vaccination with the DR3-Ag85A tetramer.
One cauda epididymis from each of the control mice (N=3) and two cauda epididymides from each of the Bscl2−/− mice (N=4) used for annexin V staining above were collected for staining the mitochondria of sperm.
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