In a second subset of pachytene spermatocytes, SUMO-1 presented as a large, densely stained, cloud-like display at one-to-five nuclear areas (Fig. 1E H).
A few stalwarts sit round a large wooden table, the light from their laptops staining the cloud of cigarette smoke that drifts above their heads.
It is characteristic for this method to create stain clouds that surround the molecules, producing a strong contrast between the background and the particle (Fig. 1a).
When images are tilted to provide the different views of the specimen needed to calculate a 3D map by the random conical tilt approach (1), these stain clouds are particularly evident (Fig. 1b).
While specimens prepared using this technique usually still adsorb in preferred orientations (Fig. 1d, insets 1 and 2), images do not have stain clouds surrounding the molecules (Fig. 1c and d).
Jay Heikes has dropped part of the ceiling in an office and, using beet juice and coffee, stained it with a purple cloud worthy of Helen Frankenthaler.
The tavern is literally covered with them -- shamrocks in different shades of green, many fading into a dark, mustard brown, stained over the years by clouds of cigarette smoke.
In a satellite image from NASA taken Friday, the dust was visible as a light brown streak staining the white clouds above Eastern Europe, according to the Weather Channel.
Typically the surface would be painted with a mesh of tiny rectangles, but the appearance of staining, of spreading clouds of colour, often earthy yellow ochres and burnt browns, the irregular lines like stitching, give her work the sense of an aboriginal art arrived at, not by imitation, but by long looking, thinking and feeling.
"The sun broke through the clouds, and jewel bright ruby, sapphire, emerald patches of light from the Chagall stained glass window shifted over her like a glimmering cloak".
An armchair he called "La Nube" (The Cloud) sits by the window overlooking the city and the sea, next to a footstool stained with the green ink that Neruda always wrote in.
