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Moreover, protein inclusions have exhibited a week stainability in the digestive gland cells of these snails as a consequence of starvation.
Such polyads would be unlikely to form viable pollen grains, thus contributing to the reduction of pollen stainability in the autotetraploid line.
oleracea L ., all male hybrids were positive for T11A and V20A, but female hybrids were not (data not shown), suggesting that the spinach male-determining factor functions in the genetic background of Group 2. The within Group 1 male hybrid progeny plants had normal anther dehiscence and high pollen fertility (∼98% stainability in Alexander's solution; Table 2 and Figure S3).
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Following genome doubling, a small, but significant, decrease in pollen stainability and in the formation of tetrads alongside a significant increase in the frequency of polyads was observed in the autotetraploid D-27.
To investigate the association between seminal hyperviscosity, the extent of semenogelin degradation, and sperm DNA integrity (DNA fragmentation index [DFI] and high DNA stainability [HDS]) in semen from infertile couples.
This change in stainability may reflect an alteration in the chemical and/or physical nature of the protoplasm, and it was the first histological indication of an altered developmental pattern.
Fissures in the cartilage layers and reduced stainability were observed in group H (Fig. 3b).
Also, increased stainability was noted in goblet cells and their mucin (Fig. 5l, m).
While a small decrease in stainability was observed from the diploid H. monacanthus (97.1%) to the autotetraploid D-27 (86.6%), an increase in stainability, meaning recovery of viability, was observed from the allotriploid S-75 (11%) to the autohexaploid D-2.3 (52%) (Table 1).
During the growing season of 2002, 2003 and 2004, flowers of each plant were morphologically characterized and pollen stainability was determined in samples of five flowers, by using 1% w/v acetocarmine and counting at least 200 pollen grains in random microscopic fields.
Histologically, stainability with safranin-O in cartilage matrices appeared to be similar in both the 500 ng BMP-7 and control groups at 4 weeks.
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