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Following electrophoresis, proteins were transferred to nitrocellulose, and the chemiluminescent signal for the heme stain was developed using the SuperSignal Femto kit (Thermo Scientific) and detected with the ImageQuant LAS4000 mini detection system (Fujifilm-GE Healthcare).
The stain was developed using avidin-biotin immunohistochemistry with a secondary anti-mouse biotinylated Ab and the Vector ABC kit according to the manufacturer's instructions, followed by the DAB reaction, which was terminated after 8 minutes.
Stain was developed using horseradish peroxidase-coupled goat anti-mouse/anti-rabbit secondary antibodies (DAKO EnVision detection kit, Dako Cytomation, Hamburg, Germany) and the Vector AEC substrate kit as a chromogen (Linaris, Germany).
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The staining was developed using DAB substrate system.
Following further washing, immunoperoxidase staining was developed using a ImmPACT™ DAB diluent kit (Vector Laboratories, CA, USA) per the manufacturer instructions and counterstained with methyl green.
pAktser473 staining was developed using Rabbit secondary (Dako).
The staining was developed using a DAB Substrate kit (Vector Laboratories).
Staining was developed using a diaminobenzidine substrate (Vector Laboratories).
Staining was developed using DAB Tablets (Sigma, Saint-Quentin Fallavier, France).
Staining was developed using liquid diaminobenzidine (DAB, Dako).
Staining was developed using Envision plus kit (Dako A/S).
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