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After 4 weeks of culture, nanofibrous scaffolds were found to contain 3 times more calcium and stronger osteocalcin stain throughout the scaffolds than the solid-walled scaffolds.
Peritubular capillary endothelia stain throughout with the CLIC4 antibody (red), colocalizing with IB4 (green), and there is punctate staining of the endothelial cell nuclei.
We consistently observed strong surface staining with SUR1 antibodies (Fig 7B) whereas SUR2 antibodies diffusely stain throughout the width of the cell in a sarcomeric repeating pattern (Fig 7C).
Briefly, testis were injected through the rete testis with Hoechst 33342 and Trypan blue (Sigma) in 3 5 μl of PBS, 1 h prior to testis dissection, to allow spreading of the vital DNA stain throughout the adluminal compartment of the testis tubules and uptake by nuclei on the adluminal side of the Sertoli cell barrier.
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Immunohistochemistry: In all empty defects there was strong type I collagen staining throughout the repair tissue.
Results: MUC4 is identified by immunocytochemical staining throughout the normal UADT mucosa, in 34 of 40 primary UADT SCC, and in 11 of 12 metastatic cervical lymph nodes.
Villus atrophy, brush border reduction and increased periodic acid-Schiff (PAS) staining throughout the subapical cytoplasm are often found in light microscopy.
In contrast, the anti human RAMP1 844 antibody coupled to Alexa 488 showed clear and constant neuronal staining throughout the whole ganglia (Figure 2B); some neurons exhibited very intense fluorescence, while others were less intensely stained; both were evaluated as immunopositive.
The dance is stained throughout with unease, the dancers propelled not as much by the music as by the rhythm of their own private pain, the music washing over them in waves of hope, irony, sadness.
Positive staining was typified by a granular, dotted brown staining throughout the cytosol.
In contrast, infected cells displayed APP staining throughout the cytoplasm by 7 hr p.i. (Figure 2B).
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