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The DAKO LSAB®2 Peroxidase kit was used to stain the expression of RARRES3 protein in tissue sections.
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We used flow cytometry to stain the surface expression of MHC class I, II molecules and CD80 and CD86 in H. pylori-infected BMDC.
When healthy human skin was stained for the expression of IP-10 no staining of the dermis was observed.
Firstly, the biopsies from untreated psoriasis lesions, non lesional skin from the same individuals and control skin were stained for the expression of the nuclear proliferation marker protein Ki67 that is expressed in cells undergoing the S/G2/M transition.
Finally, the tumors were stained for the expression of P-gp, a drug efflux transporter, for which erlotinib and afatinib are known substrates [27,28].
Cells were then collected and stained for the expression of human CD56 and IFN-γ.
Thymocyte and lymphocyte suspensions were prepared and were stained for the expression of cell surface markers as described [48].
After flow-sorting SP and non-SP (NSP) cells, the sorted cells were stained for the expression of various epithelial and non-epithelial markers (Table 2).
NK cells were derived from these donors, expanded ex vivo and stained for the expression of NKG2D, NKp30, NKp44 and NKp46.
In parallel, cells were stained for the expression of the co-stimulatory molecules CD80 and CD86 as markers of cell maturation to confirm activation of the cells (Fig. 2A).
Spleens sections from Wt L.m-OVA challenged mice (6 h) were stained for the expression of B220, CD8, CD4 (Figure 6A); CD11c, L.m antigens (Figure 6C) and CD8, IFN-γ (Figure 6D).
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