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(i) Stain Preparation Procedure.
This situation is illustrated by a negative stain preparation of yeast proteasomes (Fig. 4a), where classification produced two projection averages (Fig. 4a, insets 1 and 2).
As discussed above, addition of glucose or glycerol to the sample and the use of the carbon sandwich technique minimize such variations induced by the negative stain preparation.
After a simple and fast negative stain preparation, the undirected, "open view" of electron microscopy allows rapid morphologic identification and differential diagnosis of different agents contained in the specimen.
The shape, the integrity of the emulsomes, and the lattice of recrystallized S-layer proteins were analyzed with a FEI Tecnai G2 20 Transmission Electron Microscope (TEM) at 80 kV equipped with FEI Eagle 4k camera (FEI Europe, The Netherlands) after a negative stain preparation.
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A. phagocytophilum was first observed in Wright-Giemsa stain preparations 5 days after preparation of cultures.
In negative stain preparations of arms, three subunits can be seen.
In negative stain preparations, virions appeared mostly spherical (average diameter 104 nm) with some filamentous particles (up to 3.3 μm in length) and contained surface projections of the hemagglutinin and neuraminidase glycoproteins.
This is in strong contrast to chemically fixed, negatively stained preparations of vimentin filaments that generally exhibit smooth bending without untwisting.
Giemsa-stained preparations of trichomonad cultures were examined using light microscopy to assess parasite morphology.
All anti-CSP DAB stained preparations used to quantify synaptic boutons were mounted in Cytoseal XYL mounting medium (Richard-Allan Scientific).
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