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Protein gels were silver-stained using GelCode SilverSNAP II stain kit according to the manufacturer's instructions (Pierce).
Gels were stained with EZBlueTM Gel Staining Reagent (Sigma Aldrich) or with ProteoSilverTM Silver Stain kit (Sigma Aldrich).
Washing and staining were performed using a hybridization, wash, and stain kit (Affymetrix) on a GeneChip FS-450 fluidics station (Affymetrix).
TRAP staining was carried out using a TRAP/alkaline phosphatase (ALP) stain kit (Wako Pure Chemicals Industries Ltd., Osaka, Japan) according to the protocol provided in the kit.
Detection and visualization of the primary antibodies were accomplished using a Histofine Immuno Stain Kit (Nichirei Corp., Tokyo, Japan) according to the manufacturer's instructions.
The live-dead fixable dead cells stain kit was obtained from Invitrogen (Carlsbad, CA).
Silver staining was done using the BioRad Silver Stain kit, according to the manufacturer's recommendations.
The arrays were then washed and stained with the GeneChip® Hybridization Wash and Stain kit (Affymetrix).
Sections were then stained with Masson's Trichrome Stain Kit for morphological observation.
The gels were stained using standard procedures with silver stain (SilverSNAP stain kit, Pierce).
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Rabbit plasma, anti-sera O, H, Vi, Gram-stain kit and other reagents were procured from Difco Laboratories.
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