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The microsporidial spores stain dark violet against a pale-green background.
If a drop of ferric sulphate (used as a chemical test in mushroom identification) is applied to the mushroom flesh, it will immediately stain dark bluish-green.
A drop of dilute potassium hydroxide placed on the cap cuticle will stain dark red to blackish, and orange-yellow on the flesh, while ferrous sulphate solution turns the cuticle yellow and then greenish-yellow.
This protocol is based on the intensity of nuclei staining: normal sperm nuclei stain lightly, while abnormal nuclei (containing damaged chromatin, i.e., either decondensed or presenting fragmented DNA) stain dark.
Viable pollen will stain dark blue with analine blue, and inviable pollen grains will not (Kearns and Inouye 1993).
Centromeres are enriched in satellite repeats (Pardue and Gall, 1970) that stain dark by C-banding (McKay, 1973).
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It looked almost asleep, lying there, the black face rumpled into the carpet and the stain, darker now, spreading around the big forepaws.
The hands and feet will stain darker than other parts of the body because the skin on the extremities is thicker.
Apply a mixture of sugar and lemon juice once the henna paste starts to dry.This will keep the paste moist for longer thus making the stain darker.
Its exterior is covered with clapboards stained dark brown.
Stained dark, it looks like a painting on canvas".
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