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To determine the staging of activation of caspase-3 following kainic acid exposure, cultures were treated and fixed over a timecourse of 1 and 6 hours.
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As in quiescent satellite cells, all three markers were found at the cell membrane and revealed the shape of the cell at this stage of activation: activated and proliferating satellite cells have a rounder morphology than when quiescent.
It has been proposed that differentiation of B cells into plasma cells requires cell proliferation, at least during an initial stage of activation during which activated B cells become differentiation-competent [ 34- 39].
Microglia easily can become activated, and the initial stages of activation are characterized by the secretion of signaling molecules, slight morphological changes, and increased production of molecules involved in immune responses (i.e., major histocompatibility complex [MHC]) as well as of TLRs (Graeber 2010).
Hibbard and colleagues [ 9] conceptualized patient activation as a hierarchically structured developmental process with four stages of activation, where individuals can progress through to become fully activated in their health management.
The diffusivity of TCR is similar in T cells at different stages of activation, indicating that this parameter is not involved in allowing activated T cells to be more responsive to antigenic stimulation.
These results show that the basic parameters of TCR dynamics on the surface of T cells are not significantly different in T cells at different stages of activation, indicating that TCR mobility is not involved in allowing activated T cells to be more responsive to antigenic stimulation.
The RTM method evaluates both seismic quiescence and the following stage of activation.
So, DPM allowed us to track the first, reversible stage of activation, when a spore maintains the attributes of the dormant state.
The presence of sequential fragment ions, a phenomenon of IRMPD, permit the comprehensive sequencing of the oligosaccharides studied in a single stage of activation.
To develop a method to evaluate spindle dynamics in living oocytes and in karyoplasts during the initial stages of activation and after pharmacological disruption of cytoskeleton.
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