Sentence examples for staggered extension from inspiring English sources

Thermostable mutants were isolated after staggered extension process (StEP) with celE from thermophilic Clostridium thermocellum performed to conduct family shuffling and overlay screening of the resultant mutant library.

Several different methods have been reported for in vitro recombination or DNA shuffling: for example, the original Stemmer method of DNase I fragmentation and reassembly, the staggered extension process (STEP), and random priming recombination.

A library of enzyme variants was constructed by staggered extension process (StEp) using the genes that code for the l-ASNases from Erwinia chrysanthemy (ErL-ASNase) and Erwinia carotovora (Ecal-ASNase) and screened using activity assays.

We carried out the affinity maturation by using error-prone PCR (EP-PCR) and staggered extension process (StEP) shuffling in tandem during the second round of ribosome display.

The selected scFvs of first cycle were subjected to PCR-based random mutagenesis by using the error-prone PCR and staggered extension process (StEP) shuffling in tandem, according to the protocols described by Cadwell [37] and Zhao[38] to generate the initial mutant ribosome library of anti-Sulfanilamides scFvs.

The first such protocol was the Staggered Extension Process (StEP, [ 30]).

Here we describe the staggered booster extension phase of the study, in which subjects in the AS03A-adjuvanted H5N1 primary group received one dose of heterologous booster vaccine (A/Indonesia/5/2005; clade 2.1) at either Month 6, 12, or 36 post-primary vaccination.

The other two turrets were staggered wing turrets amidships.

In this paper, we review and construct fifth- and ninth-order central weighted essentially nonoscillatory (WENO) schemes based on a finite volume formulation, staggered mesh, and continuous extension of Runge Kutta methods for solving nonlinear hyperbolic conservation law systems.

This form is discretized on a standard staggered Yee grid, while the extension to infinity is modeled via a recently developed global complex scaling method.

In this work we modify and extend this component-wise central differencing based procedure in two directions: (1) Point values, rather than cell averages are used, thus removing the need for staggered grids, and also making the extension to multi-dimensions quite simple.