Sentence examples for staggered ends from inspiring English sources

To investigate whether this effect was specific for the type of DSB induced, we used another restriction enzyme (SacI) that creates staggered ends, again in the context of transcription.

Thus, SNPs observed in at least two overlapping reads with staggered ends are considered most likely authentic and selected for the next step.

Mboumar-9 and Mos1 transposases specifically cleave their own IR sequence to produce staggered ends but have minimal cross-reactivity on the other's IR.

A double stranded (ds)RNA is formed that is subsequently processed to 21 24 nt long dsRNA fragments exhibiting staggered ends with 3′ overhangs.

Thus, the apparent reduction in docked vesicles further than 133 660 nm is caused by the staggered ends of the active zones rather than a reduction in the density of docked vesicles.

The key modification was that by choosing restriction enzymes that generated blunt ends fragments rather than ones with staggered ends, the custom enzyme-specific adaptors used in the original protocol [ 18] could be replaced with standard Illumina Y-adaptors.

Subsequently, the DNA was digested with 4 U of the methylation-insensitive isoschisomer PspAI (Stratagene, La Jolla, CA, USA), leaving a staggered end to which a specific adaptor was ligated.

The intron endonuclease recognizes and binds to a 19-bp DNA sequence flanking the S516 rDNA site and cleaves the DNA generating a five-nucleotide 3' staggered end [ 24, 25].

Klenow enzyme was used to fill in or digest back-staggered ends produced by restriction enzyme digestion, prior to ligation with linkers.

The D10A Cas9 nickase, directed by a pair of gRNAs targeting opposite strands of target locus efficiently creates a staggered-end DSB that is recombinogenic.

The Hi-C method adds a unique step after restriction digestion, in which the staggered DNA ends are filled in with biotinylated nucleotides (as shown by the pink dot).