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Expression profile comparisons between developmental stages were performed using Significance Analysis of Microarrays (SAM) software [ 15].
Statistical assessment of annotation differences between lactation stages were performed using all expressed genes as background and Fisher's Exact Test for multiple test correction in Blast2GO.
Comparisons between RIFLE classes or AKIN stages were performed using analysis of variance and the chi-square test for continuous variables and categorical variables, respectively.
Relative warps analyses of standardized shape data at the three ontogenetic stages were performed using PCAGen (IMP-software) and the angles between subspaces defined by the first two PCs were calculated by SpaceAngle (IMP-software).
Phenotypic analyses of BrdU-positive cells in the stated differentiation stages were performed using multiple-stained series on sections 480 μm apart, also covering the entire hippocampus, using a spectral confocal microscope (TCS SP2 and TCS SP5; Leica, Nussloch, Germany).
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Augmentation procedure in two stages was performed using allogeneic and autogenous bone grafts in different areas of the osseous defect.
The training and evaluation stages are performed using the Ole Jensen dataset [33] and its mirror images.
Analysis of differential expression between consecutive developmental stages was performed using a Bayesian approach [27], [28] as implemented in the Limma package (www.bioconductor.org).
Our gene expression profile analysis of the larval developmental stages was performed using a microarray platform.
Species identification of larval stages was performed using the morphology key by Śliwińska et al. (2006) [ 56].
Time recording in the training and test stages was performed using stopwatches and the experiment was blind to the researchers who performed it.
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