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In this study, first, rice samples in various developmental stages were extracted using chloroform and water, and both hydrophobic and hydrophilic extracts were analyzed by GC MS.
Homologs were identified and their expression profiles in ovary, testis, somatic tissues, and at various developmental stages were extracted to infer function in C. gigas.
RNA samples from an assortment of 89 tissues across different developmental stages were extracted using TRIzol method according to the manufacturer's instructions (Invitrogen, Foster City, CA, USA).
Total RNA was extracted from leaf, root, stem, anther and pollen using TRI Reagent Solution (Ambion), and total RNA of seeds at different stages were extracted with RNAqueous™ (Ambion).
Total RNA of individual samples from different grain-developmental stages were extracted with TRIzol Reagent according to the manufacturer's instructions (Invitrogen), and the purification of mRNA was performed, as described by Li et al. [ 63].
RNA from cotton leaf, flower, stem and seeds at different development stages were extracted according to Wu et al. [ 32] and RNA from cotton root was isolated using Qiagen's plant Mini RNA kit.
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Total RNA from different fiber developmental stages was extracted using the CTAB-sour phenol extraction method [ 54].
Fluorescein Na captured on the actuator, induction port, and stages was extracted with methanol (HPLC grade).
Expression profile of parasite proteins for merozoite, ring, trophozoite, and schizont stages was extracted from PlasmoDB and mainly from three studies published earlier.
RNA from adult and/or embryonic vertebrate (D. rerio, X. tropicalis, A. carolinensis, G. gallus, and M. musculus) tissues and different amphioxus (B. lanceolatum) developmental stages was extracted using RNeasy Mini Kit (Qiagen), and retrotranscriptions were done using SuperScript III Reverse Transcriptase (Invitrogen), according to manufacturer.
Leaves from plants in the late rosette stage were extracted with the Ariel method for DNA extraction for quantitative PCR and cloning of chromosomal sequences.
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