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These results indicate that no significant differences in breeding parameters among colonization stages remained after accounting for phylogenetic variation.
However, the developmental rates at the 2-PN and 2-cell stages remained significantly lower in the miR-dKO group, as compared to the WT control group (Table 1).
With three different RNA extraction methods (tri reagent (Euromedex), RNeasy kit (QIAGEN) and MasterPure™ RNA Purification Kit (Epicentre Biotechnologies)), the RNA profile of GDR from the ovulation stage and from the following embryonic stages remained degraded (data not shown).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com