This allowed definition of the stage specificity of transcript expression with significance.
To trace the fate of the transcripts identified from apical or basal cells in the subsequent development stages, hundreds of transcripts from the two cDNA libraries were examined in global-stage proembryos and heart-stage embryos.
Furthermore, in the later stages of maturation, transcripts and activity of MDHAR began to increase markedly while DHAR activity and its capacity for AsA biosynthesis showed a clear decline.
In some diagrams of this study (Figs. 2, 4) we describe developmental and regional stages of target transcripts relative to their expression in P120 ventricular probes (=1) using the ΔΔ CT method.
Interestingly, many of the initial changes in the transcript profile during the early stages of interaction represent transcripts involved in metabolism, cell rescue/stress related responses and protein fate.
In the late stages of flower development, transcripts of GmMADS28 were detected in the stamens, petals, and ovules.
In comparison with the three stages of WT anthers, transcripts have differentially degrees of reduction in three corresponding stages of mutant anthers.
In the early stages of flower development, transcripts of GmMADS28 were localized in the floral meristem, and in the stamen and petal primordia.
Many of the sex and gamete function processes do not begin at such early stages of development and transcripts of such genes, presumably, would not be present (or present in very small numbers).
In order to identify genes with potentially important roles in specific stages of berry development, transcripts with a dynamic pattern were identified from within the first 20 PTM algorithm-defined profile groups.
