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Cultured stromal cells are a mixture of cells at different stages of commitment and expansion capability, leading to a heterogeneous cell population that each time can differ in the potential to form in vivo bone.
Thus the free-floating culture system contains a mixed population of cells at various stages of commitment with this heterogeneity increasing with sphere size.
To examine how α globin expression changes during hematopoiesis, we studied primary hematopoietic progenitors at various stages of commitment and differentiation.
Our study suggested that resveratrol treatment significantly increased the mRNA level of sFRP2, whereas nicotinamide treatment significantly suppressed the mRNA level of sFRP2 in the early stages of commitment of MSCs.
Early stages of commitment to neural cell fates and proliferation involve basic helix-loop-helix factors, while homeodomain proteins provide patterning and neuronal fate specification (Goridis and Brunet, 1999; Guillemot, 2007; Smidt and Burbach, 2007).
3) Author Comment: "Because PIWI-1 labels stem cells and immediate post-mitotic descendants, we consider 'progenitors' to be cells that are PIWI-1+[tissue-specific marker]+, to mark cells in the early stages of commitment".
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The model identifies three stages of commitments at each level: openings, opportunities and obligations.
Another function of rules relating to offer and acceptance is to enable the parties to understand and to mark when their discussions pass from an exploratory stage to the stage of commitment.
The decrease in T cell development in the thymus could be due to aging effects at the stage of commitment to lymphocytes.
Furthermore, given the potential heterogeneity of neural progenitors in terms of stage of commitment and potentiality, it would be advantageous to specifically isolate or enrich bone fide neural stem cells.
Thus, different types of breast cancers might originate from such stem or progenitor cells at a given stage of commitment and differentiation, as observed in hematological malignancies [4], [5].
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