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This is in contrast with a preliminary gene expression analysis of stages isolated at or past peak patency (Ramakrishnan et al. unpublished).
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In total, 12 isolates were selected for solid state fermentation, but in later stages, isolate RPW 1/1 was lost during experimentation due to unavoidable reasons.
Analysts say that the nation's dismal security situation has allowed militants to go beyond simply staging isolated terror attacks.
The key idea is that the first stage isolates a part of the variation in C in that is uncorrelated with (varepsilon_{in}), thereby overcoming problems such as reverse causality and omitted variables and achieving consistent estimation.
Flowering stages were isolated from all stages at positive side of both the PC and milky grain stages into positive side of PC1 and negative side of PC2.
Five stages of embryos representing globular, heart, torpedo, cotyledon, and mature stages were isolated from developing seeds.
Total epidermal RNAs of each molting stages (C1, C2, D1, D2, D3, E1, E2 and F; 8 stages) were isolated from one larva of each silkworm strain, using TRI reagent (Sigma) according to the manufacturer's instructions.
DOI: http://dx.doi.org/10.7554/eLife.04581.009 The periodic structure was only observed in axons, but not in dendrites, during early developmental stages, whereas isolated patches of periodic βII spectrin patterns were observed in dendrites during later developmental stages.
Blood stages were isolated as previously described [52].
Zebrafish RNAs of selected embryonic stages were isolated by TriSolution Reagent Plus (GeneMark, Atlanta, Georgia, USA).
Total RNAs of gonads at the different artificial sex inversion stages were isolated from 14 individuals of red-spotted grouper [42].
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