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A new flower opens every two to three days along the stem facilitating collection of staged tissues.
Assessment of disease-related miRNAs in human retinal diseases obviously is limited by the availability of appropriately staged tissues from patients having the same disease and causative gene mutation.
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We next examined PAD2 mRNA and protein levels from the same staged tissue extracts by qPCR and western blot in an effort to corroborate our IHC findings.
qRT-PCR was performed on cDNA synthesized from RNA samples extracted from rice cultivar Kitaake reproductive stage tissues.
The OsADF genes showed distinct and overlapping gene expression patterns at different growth stages, tissues and abiotic stresses.
Multiple developmental stages, tissues subjected to a/biotic or nutrient stress, developmental stages under stress, and resistant genotypes have been used to elucidate various genes/pathways operating in the desired situation.
Results from the advanced tumor stage tissues are reported for completeness in Appendix S2C.
Furthermore, it provides information about metabolite quantification, making it possible to make comparisons between developmental stages, tissues, or environmental conditions.
Usage frequencies of each transcription start site varied depending on plant genotypes, developmental stages, tissues, and pathogen infection.
To identify miRNAs that are associated with different development stages, tissues from both vegetative and reproductive phases were used.
Sub-categories include antibodies, strains, cell lines, developmental stages, tissues, RNAi reagents, microarray chipsets and recombinant constructs/vectors.
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CEO of Professional Science Editing for Scientists @ prosciediting.com