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The root is an ideal model for development because temporally staged samples are easily obtained by cutting further back from the root tip, and distinct cell and tissue types are observed radially outward from the root center (Fig. 2A).
To ensure that equivalent staged samples and developmental stages were taken as replicates, individual plants were sampled with the same height at each stage.
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Typical two stage samples to achieve representativeness would include over 100 local level sampling points.
At the initial stage, samples were degassed for 3 h at 300 °C.
In the first stage, samples in sealed bottles were kept at 37°C for one week.
Notably all 5 of the proteins were statistically significantly elevated in the early stage samples.
Dukes D stage samples were analyzed on HGU133plus2.0 microarrays.
At this stage samples were stored at -20°C.
All the maturation-stage samples and all the secretory-stage samples were placed into non-overlapping groups.
One of the samples in the advanced stage clustered along with the early-stage samples and two advanced-stage samples clustered separately, and closer to early-stage cluster.
Embryonic dataset (GSE22182) was download from Gene Expression Omnibus (GEO) which include four oocyte samples, eight 2-cell stage samples, six 4-cell stage samples, and six 8-cell stage samples.
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