Analysis was undertaken using Framework, a two staged "matrix based method for ordering and synthesising (qualitative) data".
Consequently, both Wortmannin and mAb13 were used to analyze a variety of staged matrix-induced cell responses such as cell morphology and various aspects of cell invasion (e.g., velocity, directionality and relative track orientation) in the second part of this study.
Moreover, this increase in directionality was significant between staged 3D matrices (P = 0.0109).
Since different cell morphologies have been associated with many tumorigenic characteristics [ 52, 53] and with a variety of invasive strategies [ 26, 27, 54], we proceeded to ask whether staged 3D matrices could differentially influence breast epithelial cellular morphologies.
In addition, although all cells were seeded as individual cells, MCF-10As seemed to cluster and invade through the staged 3D matrices as aggregates or groups consisting of several cells (Additional file 1; Movie 1).
Three different aspects of staged 3D matrix induced single cell invasion, velocity, directionality and relative track orientation, were measured in response to the presence or absence of PI3K and beta1-integrin inhibitors (Wortmannin and/or mAb13, respectively).
Next, we decided to analyze the staged 3D matrix induced invasive characteristics of MDA-MB-231 cells while questioning the roles that PI3K and beta1-integrin play in these invasive behaviors.
Therefore, our observations imply that "staging" stromal matrices, analogously to clinically relevant "tumor staging," might be an important step in assertively selecting invasive drug inhibitors.
In "Stage Matrix 1" small disturbances — a mirror that flips down underneath the table, or the shot glass's being dropped through a hidden hole in the table and into a cylinder of water below — become minor events.
This has brought him to Dance New Amsterdam for "Stage Matrix 1," a collaboration with the Austrian filmmaker and photographer Markus Schinwald that was presented by the theater and the Performa 07 biennial, which concludes on Tuesday.
