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Genomic DNA was prepared from single immature L3 or L4 larval staged individuals using the prepGEM Insect kit (ZyGEM), following standard protocols [ 108].
In the second generation, 48 L3 or L4 larval staged individuals per population and experimental regime were PCR genotyped at the fog-2 locus (see below).
It is hypothesized that differences in the genetic make-up of tumours may be partially responsible for the differences observed in survival among same staged individuals for this disease.
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Instead of staging individual runway shows, the emerging brands shared an hour of time at Bryant Park at an estimated third of the cost.
In the second stage, individuals choose one of the outstanding contracts in the market.
At the initial stage, individuals rely on mediations to complete tasks.
At this stage, individuals increase consumption of healthcare because health insurance has reduced the price of healthcare.
At any stage, individuals adhering to the physical activity guidelines are likely to be no more than 48 h from their last bout of physical activity.
In the infancy stage individuals do not have a psychological contract with the firm and the probability of exit is higher.
In licensing stage, individuals who are directly affected by any licensing action involving a facility producing or utilizing nuclear materials have rights to ask for public hearing.
To date, methods of examining the vertical distribution of reef fish larvae either under-sample late stage individuals, or are incapable of discretely sampling the water column.
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