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This has included using precisely staged animals and standardized recording conditions in which the positioning of the electrode on the pharynx was checked and recordings only showing ≤300 µV noise and <100 µVmin−1 baseline drift were used.
After, mixed staged animals are placed on each treatment plate and allowed to reach gravid adulthood.
We attempted to amplify and clone each of the three odd paralogs from Nasonia cDNA to look at this, using cDNA from staged animals (embryo, larva, adult).
We would minimally expect direct in vivo observations of Lin28 protein stability in the appropriate ced3 genetic background in properly staged animals.
In order to determine whether Dis3KD fly phenotypes were a consequence of disrupting developmentally regulated RNA turnover, we performed RNA deep sequencing analysis on total RNA isolated from developmentally staged animals.
ChIP-chip data for LIN-54 (in mixed staged animals) were obtained from GEO under the accession ID GSE28852 (Tabuchi et al. 2011); LIN-54−bound genes were determined as described in Tabuchi et al. (2011).
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The competition rules prohibit the use of staged animal models.
"Pedro Martínez and Juan Marichal exhibited appallingly bad judgment in participating in a staged animal fight," Wayne Pacelle, the president and chief executive of the Humane Society of the United States, said in the statement.
Speaker Stumbo once declared that he wasn't sure cockfighting should be illegal despite the fact that most states banned this staged animal combat in the 1800s.
Because of a weak state anti-cockfighting law and no federal enforcement of the ban on fighting animals in the Bluegrass State, Kentucky cockfighters have staged animal fights with impunity.
By staging animals in this manner, temporal variability in gene expression was minimized.
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