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The β/z males used in the Parental stage were selected from families of F3 in which no mutation of detectable lethality was found (i.e., the percentage of z/z offspring is normal).
The 8,830 healthy individuals included in the validation stage were selected from the DFTJ-cohort excluding the initial 1,461 subjects and had no diagnosed chronic diseases such as cardiovascular disease, cancer, and gout et al. The detailed information about the GWAS population and the replication samples is shown in (Additional file 1: Table S1).
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Berries from five stages were selected from three plants (3-33, 2-41, and 2-42) of the O'Neal variety of southern highbush blueberry V. corymbosum.
Five stages were selected for profiling from the leaves from leaf opening to onset of senescence, which we refer to as L-I through L-V throughout this experiment.
After euthanasia, a representative specimen from each stage was selected for μCT scanning at a 45 µm resolution (μCT, eXplore RS Micro CT System, GE Healthcare, United Kingdom).
However, because of limitations in the number of cases in our cohort (i.e., recurrence in stage I) and limitations in availability of plasma samples, only nine patients with stage I (one recurrence), approximately 10 patients with and without recurrence from stages II and III, respectively, and 8 patients with stage IV were selected from the whole cohort.
In the second stage, households were selected from every block by systematic sampling.
The late stage biomarkers were selected from the Dukes C patients and Dukes D patients.
The early stage biomarkers were selected from the Dukes A patients and Dukes B patients with mRMR and IFS methods.
At the first stage, children were selected from 21 ASMs in Kayseri by stratifying according to the socio-economic levels of their parents.
In the second stage, dwellings were selected from study EDs using systematic probability sampling which ensured that within study EDs each dwelling had the same probability of being selected.
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