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Rice at 14-day-old stage is very fragile and sensitive to abiotic stresses and has been used in many studies (Ferdose et al. 2009; Hua et al. 2009; Gao et al. 2011; Zhou et al. 2011; Hue et al. 2013); hence this stage was selected for experimentation.
After euthanasia, a representative specimen from each stage was selected for μCT scanning at a 45 µm resolution (μCT, eXplore RS Micro CT System, GE Healthcare, United Kingdom).
Second, the larval stage of EMS treatment needed to be adjusted: L3 stage was selected.
A pre-maturity stage was selected for the sampling day to analyse expression changes at the late ripening phase.
For each culture positive sample, at least one negative sample from the same week and production stage was selected.
Because gonad morphology is defective in hda-1 mutants, the appropriate stage was selected based on developmental timing of control animals.
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The second stage was sampling addresses within wards and the third stage was selecting an individual randomly from each address.
The number of features that are used in each stage is selected empirically.
The group, that contains the note with the largest value at the output of a PIC stage,, is selected.
In addition, two small East African goats in the mid lactation stage were selected in each herd for milk yield measurements.
Statistically-determined differentially expressed genes (Benjamini p-value of <0.05) at each transitional stage were selected for further analysis.
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