To illustrate how the network properties change over time, we visualize two networks from mid-embryonic stage (time point 15) and mid-pupal stage (time point 45) in Figure 3b and 3c respectively.
All five strains also clearly segregate into two clusters based on the stage (time point) of fermentation.
Our experimental setup contrasted gene expression in the dauer stage (time point 0 hour) with the dauer-exit samples (time point 12 hour) via a common reference sample (mixed stage) for each species.
To capture first transcriptional differences indicating a commitment to specific cell fates, it is important to analyse gene expression patterns at different cell stages (time points in the differentiation process).
For this purpose, we defined 7 different developmental stages (time points) starting with the imbibed seed, covering germination and continue with radicle protrusion, hypocotyl elongation, emergence, and development of the unifoliolate (see Figure 1).
In fibers of the Li 2 mutant, the expression levels of GhCSD1 remained significantly lower compared to WT fibers during elongation stage time-points (5 - 12 DPA) and remained at a seemingly basal level of expression with no change in transcript abundance in mutant fibers over time-points normally associated with elongation and SCW biosynthesis from 5 - 20 DPA.
These tests address a single (often late-stage) time point, at which moment little can be done to reverse damage (e.g., in the case of cancer).
mRNAs encoding CD36, lipoprotein lipase, and VLDL receptor were significantly reduced in 12-month-old HCM hearts, an end-stage time-point at which HCM ventricular contractile dysfunction, mass, and chamber dilation are maximal (Fig 1A,B, Fig S1).
Dynamical network biomarkers (DNB) show time-dependent alterations of network biomarkers monitored and evaluated at different stages and time points during the development of diseases [12].
