Figure 3 Two-stage sphere decoding.
A stage or sphere of existence, then, is a fundamental project, a form of life, a mode of being-in-the-world that defines success in life by its own distinctive criteria.
At this stage, the spheres gave rise to circular clusters of cells, many of which migrated from the clusters to the surrounding areas.
In contrast with previous results, they observed the expressions of both miRNAs and piRNAs are firstly increased and then decreased, with a peak expression at sphere stage for miRNAs and shield stage for piRNAs, respectively [ 19].
To culture primary germ layer progenitor cells, embryos were manually dechorionated in E3 buffer at sphere stage (4 hpf) or 75% epiboly stage (8 hpf).
Western blot detection showed that the zygotic zWnt4b protein was translated at sphere stage and its peak was brought from shield stage.
Embryos injected with MOs were dechorionated and mounted in 0.8% LMP agarose at the sphere stage for lateral cell observations, or at the shield stage for prechordal plate cell observations.
The cells were sucked from the prospective mesodermal region of a donor at the sphere stage, and transplanted into the same position of wild-type host embryos at the same stage.
Based on a two-dimensional framework (project stage and analytical sphere), a structured and integrative synthesis of relevant studies is outlined.
Fertilized and normally developing embryos were selected under a stereomicroscope (×8 to × 50) at 4 h post-fertilization (hpf) (i.e., the sphere stage of the blastula period) and used for exposure experiments.
The Marangoni effect switches the states of a "long" bubble during the pulse duration stage and a sphere or a circular bubble during the pulse off stage, exporting the pulse pressure and yielding a large convection heat transfer at the vapor liquid interface.
