Two-stage regulation mode was adopted in the main circuit of the power.
Compared to the prokaryotes, the complexity of multi-stage regulation in eukaryotes, involving fast reactions (dimerization, protein DNA binding/unbinding) and slow reactions (transcription, translation, degradation, multiple transcription factor cascades), is higher, and hence have been postulated to be a significant source of delay in the subsequent steps of the reaction [31], [39], [40].
Multi-stage regulation at transcriptional and post-transcriptional levels contributes to reprogramming protein synthesis.
These findings argue for a two-stage regulation of Sst expression: an early SATB1-independent phase, which denotes subtype commitment of SST+ interneurons, and a subsequent SATB1-dependent phase, which reflects the maturation of SST-specified MGE precursors into functional interneurons.
This inconsistency between mRNA (transcription) and protein (translation) may be due to the complexity of the gene's expression or to multi-stage regulation mechanisms of the gene, which may be correlated with more efficient clearance of mRNA species (i.e., mRNA degradation).
We also observed that genes with the highest levels of protein production were more likely to exhibit stage-regulation (Additional file 2: Figure S10).
Genes with upstream ORFs had lower mean translation efficiency, but no evidence was found for involvement of uORFs in stage-regulation.
Thus, we were unable to find convincing evidence that the putative uORFs contribute to stage-regulation of translation between PCF and slBF.
This data does not rule out the possibility that some uORFs confer a component of stage-regulation, which could be revealed with further refinement of the transcriptome and translatome or by examination of other developmental stages.
