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Final assembly takes place in California, giving us easy access to final stage quality control.
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This two-stage quality control process ensures the accuracy of the data.
Previously, we have proposed a three-stage quality control [ 22] strategy for exome sequencing analysis that emphasizes the need to implement quality control at all stages of exome sequencing processing: raw data, alignment, and variant calling.
Amongst the up-to-date disease-associated regions (http://www.immunobase.org/downloads/regions-files-archives/2014-05-04, archived on 04 May 2014), 545 unique non-MHC autosomal regions were initially included and 341 regions were used for colocalisation analysis after multi-stage quality control.
The second stage performs quality control, rejecting frames that may be erroneous according to several criteria.
The first stage of quality control was performed locally, based on the two data files generated through double data entry.
Samples that did not pass this stage of quality control were excluded from further analysis (n = 3).
The second stage of quality control was performed centrally at the Estonian site, by double-checking each local dataset, attempting to detect other errors, such as incompleteness (missing values), inconsistencies (incorrectly followed skip-outs), irregularities (numbers inserted in text variables), and out of range data (i.e. very large number of siblings or sexual partners).
Actually, human, chemical and colour analysis are performed in key production stages for quality control.
Pre-processing consisted of five initial stages of quality control.
The ECORN-CF patient advice platform is designed to include a two-stage central quality control mechanism.
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