Ovaries (n=2 per stage, pooled) were collected from Tg females aged 2, 4 and 6 months.
For each developmental embryonic stage, pooled read counts were used for recalculating RPKM values.
At this stage, pools of 6 individuals were randomly constituted for hemolymph and visceral ganglion samples.
RNA was extracted in TRIzol (Invitrogen, Carlsbad, CA), from adult animals and 20-h larvae (phylotypic stage) pooled from several females.
In the first mapping stage, pooled sequences were analysed genome-wide for intervals displaying relativereduction in diversity between phenotype pools, and candidate genomic loci were identified for influence upon parasite infection levels.
Observed heterozygosity was less than expected for adults and at each life stage pooled across the five trees, indicative of a slight heterozygote deficit at all stages in this population.
In the initial biomarker-screening stage, pooled serum samples from 20 AMI patients and 20 controls underwent Solexa sequencing (See Additional file 1: Table S1) to identify miRNAs that showed significant differences between the AMI cases and matched controls.
Atlas's previous fund was a $350 million earlier-stage pool of capital that it announced in 2017.
Each replicate was harvested at the same time of day and consisted of seed samples at four developmental stages pooled from three plants.
Despite the fact that microRNAs hsa-miR-494 and hsa-miR-21 share important targets such as PTEN, this combination was not significant after the log-rank test for CSII or all clinical stages pooled together (data not shown).
