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The developmental stage of testes was determined according to the classification worked out for oviparous teleosts by Grier (Grier, 1981) and modified by Kinnberg et al. (Kinnberg et al., 2003), while the developmental stage of oocytes was determined according to the classification worked out for the guppy, Poecilia reticulate, by Takano (Takano, 1964) and modified by Koya (Koya et al., 1998).
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However, no such relationship was detected in the developmental stages of testes (Fig. 4).
The developmental stage of the testes was that of sexually mature males with the final half of the testes full of spermatozoa (pearl white colour) and the anterior part with active meiosis (transparent white colour).
We assigned scores and evaluated them based on the total number of abnormalities in each of the following three tissue types: a) testes, b) ovaries/oviducts in the presence of testes (number and developmental stage of female gonadal tissues found in the presence of testes is considered a measure of the severity of the intersexed condition), and c) Bidder's organs.
This was the most advanced stage of GC development in WT testes at d21; however, the number of elongated spermatids (and thus the total number of spermatids) was also reduced in adult PTM-ARKO testes (Table 4).
The use of testes at various developmental stages highlighted several gene activation or repression patterns throughout the reproductive cycle.
Importantly, the number and developmental stage of female gonadal tissues found in the presence of testes are considered a measure of the severity of the intersexed condition.
To identify new regulators of chromatin remodeling involved in the switch from a histone-based chromatin to a protamine-based configuration, we analyzed postmeiotic-enriched transcripts of stage-specific testes transcriptome data published by Vibranovski et al. (Vibranovski et al., 2009) with a particular focus on genes conserved in mammals.
The gonad integrity as well as the maturity stages of ovaries and testes are diagnosed microscopically, and the gonado-somatic index (GSI) in trout and chub is determined according to [39].
We previously performed transcriptional profiling of testes taken from N. vitripennis pupae in the yellow body-red eye developmental stage (Akbari et al. 2013).
Further phenotypic analysis reveals that Leydig cell differentiation is arrested at the progenitor cell stage in the testes of null mice.
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