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At each stage of staining, whole tumor sections were imaged using a robotic fluorescence microscope, as previously described [ 40, 41].
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Over the 49-day staining period, different patterns of change in tissue contrast were observed in earlier and later stages of staining and after the input of additional iodine.
Sequential processing steps involved in the diagnostic preparation of tissue--gross dissection, selection of tissue blocks, processing, imbedding in paraffin, cutting and mounting on glass slides, all funnel through the final stages of staining, and cover-slipping in preparation for review and diagnosis by a pathologist.
The hairs were examined by light microscopy to evaluate nuclear staining in terms of its presence/absence, site of staining and stage of hair.
In contrast, the frequency of p53 accumulation increased with grade and stage as did the frequency of staining for the proliferation marker Ki67.
First, registered patients in the KBCR database were heterogeneous with respect to breast cancer stage, IHC staining results, and presence of comorbidities.
Cells early in apoptosis positively stained for Annexin V, whereas cells in the later stages of apoptosis stained positively for Annexin V and PI due to late apoptotic cells having leaky plasma membranes (magnification = 400×).
Laboratory diagnosis of TB remains in a stage of acid-fast staining.
Whenever a PrImR average expression pattern was not available for the gene and/or the stage of interest, specimens stained with the gene of interest and tyrosinated tubulin were inspected under fluorescence microscopy.
Moreover, at later stages of development, staining was also apparent in interfascicular fibers suggesting that ESK1 expression occurs in tissues undergoing or that will undergo secondary cell wall deposition and not specifically in TE cell lines (Figure 5).
(A - C" ) Cross section (ZX view –see reference axis in Figure 1K ) of the dorsal epidermis of embryos at stage 14 stained for D Patj (green) and Yrt (fire LUT-pseudocolor).
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