At the initial stage of coloration, injected electrons are captured mainly by the W6+ centers that have no such bonds.
All neurons in the advanced stages of apoptosis, injected or non-injected, showed strong general binding of anti-MANF antibodies that was most probably nonspecific (not shown).
Previous studies have reported that an RNA element within the gurken protein coding region, called the GLS (gurken localization sequence), is both required and sufficient for transient (e.g., stage 8/9 only) localization of injected gurken transcripts to the AD corner of the oocyte [20].
To confirm the embryos stages of the injected embryos, an ISH for somite marker myod was performed, and is shown in Figure S3A-F (dorsal views), Figure S3G-L (lateral views).
Axolotls with limbs at the midbud stage of regeneration were injected i.v. with either DiI-liposomes or DiI + clodronate(clodro)- liposomes, 36 hr prior to implantation of normal or senescent nGFP+ cells into contralateral regenerating limbs.
Tomato fruits were harvested at the mature green stage of development and injected with a buffer solution contained 10 mM MES, pH 5.6, sorbitol (3% w/v) and 100 μM of ACC, or IAA, or IAA + ACC (100 μM each), or PCIB (all Sigma-Aldrich products).
Stage Six: Over-flush stage: inject 8%% NH4Cl.
To examine the biological function of the puf-A gene, zebrafish embryos at the 1∼4 cell stage were injected with one of the two puf-A-MO antisense oligonucleotides, MO1 and MO2 (see "Materials and Methods" and Fig. S1 for locations of target).
To determine the optimal concentration of PhiC31o mRNA enabling efficient targeted transgenesis of DOI at zygote stage, we injected a solution containing various concentrations (from 0 to 45 ng/μl) of PhiC31o mRNA and 10 ng/μl of pBER donor vector into fertilized eggs carrying a heterozygous targeted allele at the Rosa26 locus.
