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However, for class I patients who have severe or moderate dentition crowding, the goal of first phase of treatment was to bring malaligned teeth into aligned, indicating that the stage of alignment would be of value and statistically improve psychological status of class I patients.
This unregistered data requires a second stage of alignment before it can be used as an analytical tool.
The wires were pulled in a distal direction in order to simulate the initial stage of alignment and leveling, when the archwires must slide through the brackets.
After a first stage of alignment using POA v2 [ 50] and MUSCLE [ 51], the alignment was refined using the clustal-W subalignment tool available in MEGA 4 [ 52] and edited by eye.
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The first stage of MUMmer alignment involves identifying alignment anchors.
Progressive alignment is further complicated by the potential to introduce errors or bias based on the phylogenetic trees selected for progressive alignment and accumulate pairwise errors at each stage of the alignment [25].
In the second stage of the alignment, putative novel junctions and 186,074 known junctions compiled from the Ensembl gene predictions and Ref-Seq gene models were combined and supplied to Tophat, which then aligned reads separately.
The acoustic levitator was mounted on an xyz micropositioning stage for alignment of the sample and a visible microscope.
The ND layer was therefore excluded during later stages of image alignment.
Up to this stage, the alignment consists of aligned representatives of NCIV clusters.
In applying this concept to clinical practice, friction might influence the force in the initial stage of leveling and alignment, when crowding is severe, or in the final stage, when using a larger diameter wire.
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