The efficacy of the methods was compared across nymphal stages, localities and habitats.
At the pupal stage, most localities exhibited peak mortality in the spring of 2012, again with the exception of Hirono, at which mortality peaked in the fall of 2011 (Additional file 5: Figure S3c).
PCR was significantly more sensitive than microscopy in all habitats, developmental stages and localities in Chuquisaca, Bolivia.
The central finding of the present study is that the PCR is significantly more sensitive than microscopy in all habitats, developmental stages and localities in Chuquisaca, Bolivia.
Finally, in order to investigate the interactions among biological processes, temporal developmental stages, and spatial locality in tissues, we generated two additional network compendia.
The SAGE sample design entails a two-stage probability sample that yields national estimates to an acceptable precision at provincial level, by locality type (urban and rural) and by race (including Black, Coloured, Asian, and White).
The SAGE sample design entailed a two-stage probability sample that yielded national and sub-national estimates to an acceptable precision at provincial level, by locality type (urban and rural) and race (including Black, Colored, Indian or Asian, and White).
For both the microscopy and PCR assays, we calculated sensitivity (number of positives by a method divided by the number of positives by either method) and discrepancy (one method was negative and the other was positive) at the locality, life stage and habitat level.
17 We used a two stage consent process: at locality manager level and for individual women attending a group for the first time.
In the present study, pH, EC, primary and secondary macro and micronutrients vary significantly across seasons, localities and stage of the crop.
