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Then, the Hepes-Ringer solution was replaced by 1.5 2 ml fresh buffer and the culture dish was transferred to the Delta T Stage holder 30 min after the animal's death.
The Ludin chamber is placed in a dedicated stage holder and the whole microscope set-up is surrounded by a temperature and <img src="http://journals.plos.org/plosone/article/asset?id=info?doi/10.1371/journal.pone.0013190.e020.PNG" class= inline-graphic"/> regulated incubating system (LIS, Basel, Switzerland).
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It would be a trivial task to complete this fluid-stage holder model by attaching the remaining fluid layer and additional silicon nitride membrane to the bottom of the model.
In-situ high resolution transmission electron microscopy (HRTEM) with a hot-stage holder was used to observe the initial reaction process between molten Ag-Cu-Ti and SiC at the atomic level.
He was able to make observations in polarized light, and his microscope was equipped with a hot stage (sample holder equipped with a heater) enabling high temperature observations.
Four persons were involved in this stage (two holders of a Master's degree in Epidemiology and two Medical doctors).
For example, in females, degree holders (ME = 0.013) and older people (ME = −0.001) were correlates only when meeting the recommended level was modelled as a single-stage process.
The sample heating temperature was measured during the heating stage of the holder using a thermocouple placed directly in contact with the sample.
This measurement method allows for the inspection of geometrical parameters such as depth and width of scratches and cracks and consists of a low-energy He3Ne red laser beam (wavelength 633 nm) with a cross-section of 0.48 mm, beam expander with special designed slit, multi-axis stage with sample holder and CCD (charge-coupled device) visual detection system.
Safina had Williams at break point down in the third game, at which stage the title-holder launched a flashing serve at 125mph.
d) Picture of the inside of the chamber containing the Attocube ANPz/x nanopositioning stack, highlighting the positioning stages and fiber holder.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com