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(J ) Luciferase assay measuring Wnt/βcatenin activity from 28-somite stage dissected tail-buds after over-expression of BATLuc and CMV-Renilla constructs and either Hoxa13mutH or Hoxa13dn, or Hoxa13mutH with Hoxc11mutH or Hoxa13dn with Hoxc11dn, or Hoxa13mutH with Hoxc11mutH and Hoxd10mutH or Hox13dn with Hoxc11dn and Hoxd10dn.
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In this stage, Dissect searches for the optimal overlap resolution according to an extended version of the overlap resolution scheme described in the previous section.
B73 maize anthers were staged, dissected and stored in RNA later.
Most whole mounted specimens were fixed in 10% neutral-buffered formalin for 48 hours, stored in 70% ethanol, and staged, dissected and photographed under a Zeiss Stemi SV 11 dissecting scope.
Randomly five seeds from each genotype at each time point (developmental stage) were dissected under the binocular stereo microscope at 1.6x magnification and pictures were taken using Axio Vision Rel.
To measure the tissue specific ethylene production, another batch of 12 fruit for each maturity stage was dissected and the different tissues were pooled per tissue type for each maturity stage.
The tiny first or second true leaves in the seedlings at 10-day-old stage were dissected and used for live-cell imaging of trichomes at various developmental stages.
Larvae were raised on fly food supplemented with all-trans retinal (Sigma, 100-200 nmol added daily on top of vials containing ∼5 ml of standard medium) until the third instar stage, then dissected in HL3 buffer [30].
For DAPI staining of pollen grains, anthers at different stage were dissected and mounted in DAPI solution (DAPI 5 µg/ml, PIPES 50 mM, EGTA 5 mM, NP-40 0,1%, and DMSO 10%) and incubated for 30' before the observation on a Nikon fluorescent stereo-microscope E800 equipped with a 100× optic.
Larvae at the late 3rd instar stage were dissected for salivary glands in 0.7% NaCl.
Nerve cords from embryos of the desired genotype and developmental stage were dissected and mounted in 70% glycerol/PBS.
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