Sentence examples for stage cultures from inspiring English sources

Cells were seeded at 105 cells/cm2 and grown for 3 days to obtain subconfluent stage cultures, at 2 × 10/cm2 and cultured for 7 days to obtain confluent stage cultures, and at 6 × 10/cm2 and grown for 10 days for over-confluent stage cultures.

We report a methodology for the reliable execution of drug pulse assays and detail a synchronisation strategy that produces well-defined tightly synchronised ring stage cultures in a convenient time-frame.

Gametocyte production of the mutant parasites was analysed in blood stage cultures that were optimised for gametocytogenesis [38].

Erythrocytic asexual stage cultures of P. falciparum strain 3D7 were maintained in culture medium (RPMI 1640, 25 mM HEPES, 10 µg/ml gentamycin, 0.5 mM hypoxanthine, pH 6.75), supplemented with 25 mM sodium bicarbonate and 10% human serum.

For example in the early stage of the cell cultures the literature shows that both NHKs and HDFs tend to express more stimulatory factors, while more inhibitory factors are produced in late stage cultures.

A comparison of the recovery stage and pre-stable stage cultures revealed significant increase in PPP and decrease in TCA cycle fluxes for the CSG+solASG condition with no significant difference in albumin synthesis rate [Table 5].

Second stage culture was initiated by inoculating 25 mL of fresh sterile liquid media with 5 10% v/v inoculum from the first stage culture.

Figure 3 Effect of pH on curdlan production in nitrogen free medium using two stage culture technique.

Briefly, first stage culture was initiated by inoculating 25 mL of sterile liquid medium with a loopful of freshly grown culture, under laminar airflow cabinet.

Hence we carried out two stage culture technique to optimize the pH that was favourable for curdlan production in the mutant strain.

Two stage culture was most widely used method to study the effect of any variable on production of metabolites (Lee et al.2001).2001