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Particles from this process were collected on Corning glass substrates, placed on a rotating stage, at room temperature and at atmospheric pressure.
Using a solvent to fuel volume ratio of 1 1 (VDES/VFuel = 1 1), the extraction efficiency reached 82.40% and 62.16% in the first extraction stage at room temperature.
These experiments were performed on the microscope stage at room temperature.
FRET efficiencies were measured from the same cells during a 2-minute control period and for 20 minutes after the addition of 100 nM rapamycin on the microscope stage at room temperature.
For simplicity, the times pf reported here were temperature-compensated to reflect the time embryos would have reached the relevant developmental stage at room temperature (21°C).
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Treatment with glutaraldehyde was performed in several stages at room temperature.
Loose patch and whole cell patch clamp recordings were done in wild type larvae at different stages at room temperature (23 24°C).
Based on the first stage experiments, T1 size coal was oxidized with air at 150 °C on a fixed bed for 4 h, xanthated following the procedure used in the first stage but at room temperature during 8 h, and stirred during 4 h.
Experiments for a single pass of the ECAP followed by four stages of the bolt forming process were carried out to manufacture a microstructure-refined bolt made of an aluminum alloy 6061 by using the multi-stage former at room temperature.
At the final stage, after cooling at room temperature over a period of 1 h, the gel was transferred into a crucible and calcined at 600 °C for 10 h to obtain ZnO NPs powder [21, 32]. 10 μL of toluene as the internal standard and 10 μL of a 5 1 (v/v) ratio of CEPS/H2O were added to 5 mL of each solvent (isopropanol, acetone, and n-hexane) representing the optimized work solutions.
Raman spectra for nanowires at different growing stages were collected at room temperature and shown in Figure 5.
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