Embryos shifted to 25°C prior to the lima bean stage arrested at the L1 stage (Table 4).
Thus, co-injection of Oct4 mRNA, but not control mRNA, partially rescued the Oct4-MO-induced multicell stage arrest phenotype.
Mitotic or reproductive death after G2 stage arrest is induced by DNA damaging agents and produces enlarged cells containing multiple nuclear fragments, readily distinguished from apoptotic nuclear fragments.
Indeed, complete frataxin deletion leads to early embryonic lethality in plants and mice [13], [14], to L2/L3 larval stage arrest in C. elegans [15], and to reduced larval viability and metamorphosis failure by systemic silencing in the Drosophila [16], [17].
Embryos injected with anti-SpRunt morpholinos develop two distinctive phenotypes: an early blastula stage arrest and a later gastrula-stage arrest.
Neither culture treated with inhibitor advanced past the early trophozoite stage, arresting in the ring or early trophozoite stages.
We assessed ROS levels at two carefully chosen time points for nematodes within each treatment group: 34 and 68 hours after L1 stage arrest at 20°C; 24 and 48 hours after L1 stage arrest at 25°C.
Whether this is a true stage arrest phenomenon elicited by the presence of S. gordonii could not be determined since cells were not a priori synchronized.
As shown in Figure 1C D, both skin cancer cell lines showed a significant and time-dependent G2/M stage arrest and a sub-G1 peak.
In late stage arrested C. elegans oocytes, OMA-1 and OMA-2 (redundant RBPs) are required for progression through meiotic prophase I [ 44].
