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"They're stage animals.
Of the cast, the two men, temperamentally very dissimilar, are the most natural stage animals.
L4 stage animals were heat shocked as described above.
Experiments were started with synchronized L1 stage animals.
L1 stage animals were synchronized by treating gravid adults with hypochlorite and incubating released eggs in M9 buffer overnight [28].
To score at the L3 Pn.pxx stage, animals were scored roughly 45 hours after eggs were plated.
Similar(9)
Individual differences in the distribution of PrPSc in CSF samples were observed in terminal-stage animals.
Individual differences in the distribution of PrPSc in spleen and cerebrospinal fluid samples were observed in terminal-stage animals.
About 30 synchronized L4-stage animals were added to each well and allowed to develop to adults, followed by the addition of FudR.
cDNA template was prepared from mixed-stage animals.
L4-stage animals were then harvested after these respective intervals and then treated exactly as above.
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