Using three-stage agitation in fed-batch fermentation, 2,3-butanediol was accumulated to 110.04 g L−1, and then converted to acetoin.
Furthermore, the two-stage agitation allows the reactor to behave as a two-phase system and the compounds in the two stages could partially be mixed.
This paper discusses computational and experimental studies to investigate flow patterns in an industrial-scale (110 m3) continuous reactor employing a three-stage agitation system with a plunging jet inflow.
To overcome these problems, a two-stage agitation speed control strategy for 2,3-BD fermentation was used.
In this work, a two-stage agitation speed control strategy was found to be more suitable for 2,3-BD fermentation by strain 10-1-A (Table 7).
After statistical optimization of the fermentation medium composition and the culture conditions, 2,3-BD production was carried out in fed-batch fermentation by using two-stage agitation speed control strategy.
With a two-stage agitation speed control strategy, 115.7 g/L of 2,3-BD was obtained from glucose by fed-batch fermentation in a 5-L bioreactor with a high productivity (2.4 g/L·h) and yield (94% of its theoretical value).
The updated production by the festival's artistic director Jochen Schönleber detracted from this poignant moment by having soldiers dart on and off stage in agitation, but generally the opera unfolded clearly.
This is a delicious scenario, then: Murray and Djokovic duking it out on the final Sunday of the championship again, with their coaches and former rivals looking down from the stands in various stages of agitation – or, in Lendl's case, appearing as if he'd just been taken out of a cryogenic ice box.
Elias Bender Ronnenfelt, the band's vocalist, stalked the tight stage with sulky agitation, shoulders hunched, barking into his fist.
