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Electrodeposited thin metal stacks were used to fabricate CZTSe thin films by selenization.
The resulting new particle stacks were used as input in the subsequent 3D auto refine and classification processes.
Prior to the inversion, near-, mid-, and far partial stacks were used to conduct seismic well tie.
These image stacks were used for cell counting or assembled into extended focus photographs; brightness, color, and contrast balanced and assembled into panels with CorelDraw 11 (Corel, Ottawa, Ontario).
The mean background determined from the intensity histogram of recorded image stacks was subtracted [36] and the resulting image stacks were used to automatically define peak regions of interest of synaptic bouton size [37], where AP-evoked fluorescence decrease (FM 1 43 and anti-synaptotagmin-1-cypHer) or increase (synaptopHluorin) occurred in difference images.
The image stacks were used for PGC counts and to score for abnormal PGCs.
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Finally, the program Populations in Stacks was used to process all SNP data across individuals.
For single-molecule FISH images, a maximum intensity projection of Z stacks was used to quantify mRNA distribution.
In the lower panels, part of the same stacks was used to remove the autofluorescence from the yolk and the vitelline in Fiji.
Brightest point projections of the Z-stacks were used for image analysis.
Non-manipulated maximum projections of z-stacks were used for quantitation (Fiji).
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