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Scanning was done at 28 μm isotropic voxel size to produce five image stacks of each femur.
The NMR structures and Autodock analysis suggest that the pocket with the most favorable amphipathic property for anesthetic binding is located between the W15 side chains at the center of the dimeric hydrophobic core, with the possibility of two additional minor binding sites between the F12 and F52 ring stacks of each monomer.
The chart showing the floor and wing location, within the stacks, of each subject classification was revised sixty-five times during construction.
To allow visualization of the arborization patterns of each of the FruM/OA neuron in three-dimensions, we incorporated the data generated from the confocal image stacks of each neuron onto a single standard brain and generated a rotating projection (Figure 4A4, B1 3 and Movie S7).
Figure 2 shows the resulting 3D renderings (using the shadowing feature) from SHG z dimensional stacks of each of these gels, where the intensities are scaled for presentation.
If you are counting money, start by making stacks of each kind of bill or coin you are counting.
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The kernel is responsible for keeping track of the top-of-stack for each of the different tasks.
Each chest contains 2-6 stacks of items; each stack may have one or several of its item.
Confocal scanning laser microscopy (CSLM) was used to create Z-stacks of each CNV by assessing the fluorescence in each layer of the CNV.
After obtaining z-stacks of each dissected specimen, individual flies were assigned as either GFP+ or GFP− for each particular tissue region.
An automated stage was used on both 7 MP and LSM780 systems to acquire multiple time-lapse recordings simultaneously, and acquire x- y tile scans and z-stacks of each of these animals.
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