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Oh, the stacked image is of the Omnia B7610 on the bottom and the Omnia Lite in the middle.
The stacked image size is 1 μm × 1 μm (scale bar is 200 nm).
Students learned the usefulness of combining (bias/dark/flat) calibration images into a "master" image and their eventual subtraction or division to the stacked image.
The stacked image is then output to file and stored as a sequence of.tif files that are viewed in.avi format (see below for the full range of.tif images).
Simple mathematical formulas were required to calculate the magnification factor of the telescope, the exposure time of the camera, and the resulting signal-to-noise ratio of the final stacked image.
Images were taken right after injection and the resulting stacked image shown in Figure 4a.
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Time-lapse images of the EB3-GFP movement were recorded every 2 sec, and 35 stacked images were composed.
Whole-mount movies and stacked images were made and analyzed using Imaris 7.6.4 software.
Dendrite ends were counted manually from stacked images for the entire field of view.
This video was achieved by "stacking" image sequences provided by NASA from the crew at International Space Station.
In other endomicroscope designs, en-face (XY-plane) imaging is performed by stacking images along the Z-axis in sequence.
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