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Confocal images are represented as the average projection of a stack of sections (Z-series) or as a single section.
Contacts can also be illustrated as they appear throughout the stack of sections as a simple 0.5 μm layer or as a transparency of all layers merged together.
Images can be illustrated as they appear throughout the stack of sections as a simple 0.5 μm layer or as a transparency of all layers merged together.
We followed the reviewer's suggestion and imaged again all used SNARE markers and integrated the signals through a stack of sections covering the entire cell.
The authors could rule out that their results are artefacts by addressing several points: 1) Integrate the fluorescence signals of the various SNARE proteins through a stack of sections covering the entire cell.
The new set of data and its quantification is now shown in Figure 6 figure supplement 3. Reviewer #1: 1) Integrate the fluorescence signals of the various SNARE proteins through a stack of sections covering the entire cell.
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This enabled defined stacks of sections to be visualized as well as the 3-dimensional stack through the entire retina to be rotated 90°.
By rotating the entire stack of optical sections 90° (Fig 1, A3 E3), we created a cross-sectional view where the cones are on the top and the inner retina at the bottom.
MacLeod and his colleagues create their designs on a computer, which renders every adornment or edible sculpture as a stack of cross sections, each thinner than a sheet of paper.
MacLeod and his colleagues create their designs on a computer, which renders every adornment or edible sculpture as a stack of cross sections, each thinner than a sheet of paper.
Along the main axis of the large second room stands a row of four white minimalist sculptures, each a nine-foot-tall stack of concrete sections, 12 inches per side.
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